Journal: The Journal of nutritional biochemistry

Article Title: Resveratrol prevents the development of choroidal neovascularization by modulating AMP-activated protein kinase in macrophages and other cell types.

doi: 10.1016/j.jnutbio.2014.05.015

Figure Lengend Snippet: Fig. 3. RSV treatment reduced the levels of inflammatory and angiogenic molecules.Protein levels of VEGF (A), ICAM-1 (B) and MCP-1 (C) were increased 3 days after CNV induction. RSV pretreatment significantly suppressed these increases. n=8. **Pb.001, *Pb.01, †Pb.05.

Article Snippet: In the samples taken 3 days after the PC, VEGF, MCP-1 and ICAM-1 levels were determined with mouse VEGF, MCP-1 and ICAM-1 ELISA kits (R&D Systems, Minneapolis, MN, USA) as previously described [10].

Techniques:

Journal: The Journal of nutritional biochemistry

Article Title: Resveratrol prevents the development of choroidal neovascularization by modulating AMP-activated protein kinase in macrophages and other cell types.

doi: 10.1016/j.jnutbio.2014.05.015

Figure Lengend Snippet: Fig. 5. RSV inhibited NF-κB and inflammatory and angiogenic molecules in vitro. ARPE-19 cells (A–C), b-End3 microvascular endothelial cells (D–F) and RAW264.7 macrophages (G and H) were stimulated with TNF-α. RSV significantly suppressed the phosphorylation (activation) of NF-κB by TNF-α (A, D and G) and the subsequent induction of VEGF (B and H), MCP-1 (C and F) and ICAM-1 (E). All of the effects of RSV were attenuated by an AMPK inhibitor, compound C. n=8. **Pb.001, *Pb.01, †Pb.05.

Article Snippet: In the samples taken 3 days after the PC, VEGF, MCP-1 and ICAM-1 levels were determined with mouse VEGF, MCP-1 and ICAM-1 ELISA kits (R&D Systems, Minneapolis, MN, USA) as previously described [10].

Techniques: In Vitro, Phospho-proteomics, Activation Assay

Journal: The Journal of nutritional biochemistry

Article Title: Resveratrol prevents the development of choroidal neovascularization by modulating AMP-activated protein kinase in macrophages and other cell types.

doi: 10.1016/j.jnutbio.2014.05.015

Figure Lengend Snippet: Fig. 7. A model for the preventive effects of RSV on CNV.RSV treatment significantly suppresses CNV development by preserving AMPK activation, which inhibits macrophage recruitment as well as the induction of inflammatory processes, including NF-κB activation and the subsequent up-regulation of inflammatory and angiogenic molecules such as ICAM-1, MCP-1 and VEGF in multiple cell types. PC, laser PC for CNV induction; Vascular EC, vascular endothelial cells.

Article Snippet: In the samples taken 3 days after the PC, VEGF, MCP-1 and ICAM-1 levels were determined with mouse VEGF, MCP-1 and ICAM-1 ELISA kits (R&D Systems, Minneapolis, MN, USA) as previously described [10].

Techniques: Preserving, Activation Assay